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control nb820 59231  (Novus Biologicals)


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    Structured Review

    Novus Biologicals control nb820 59231
    Control Nb820 59231, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+nb820+59231/Human+Kidney+Whole+Tissue+Lysate+(Adult+Whole+Normal)/pm41192745-152-12-12
    Average 94 stars, based on 10 article reviews
    control nb820 59231 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Positive Control:

    Article Title: Modeling CubAm function and regulation in proximal tubular cells using iPSC-derived kidney organoids.
    Article Snippet: Cells were lysed in SET buffer (10 mM Tris–HCl pH 7.4, 150 mM NaCl, 1 mM EDTA and 1 % SDS) and the protein concentration was quantified by the BCA assay (#23225, Thermo Fisher Scientific). .. Twenty microgram of human kidney whole tissue extract as positive control (NB820-59231, Novus) and 50 μg of whole cell extracts were resolved by sodium-dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in 7.5 % or 12.5 % (vol/vol) polyacrylamide gels. .. The gels were transferred to polyvinylidene difluoride (PVDF) membranes, blocked with 5 % skim milk (42590.01, Serva) diluted in PBS-T (1x PBS, 0.1 % Tween 20) and probed overnight at 4 ◦C with sheep anti-cubilin (dil. 1:2000, AF3700, R&D Systems), goat anti-amnionless (dil. 1:2000, AF1860, R&D Systems) or mouse anti-actin (dil. 1:5000.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Modeling CubAm function and regulation in proximal tubular cells using iPSC-derived kidney organoids.
    Article Snippet: Cells were lysed in SET buffer (10 mM Tris–HCl pH 7.4, 150 mM NaCl, 1 mM EDTA and 1 % SDS) and the protein concentration was quantified by the BCA assay (#23225, Thermo Fisher Scientific). .. Twenty microgram of human kidney whole tissue extract as positive control (NB820-59231, Novus) and 50 μg of whole cell extracts were resolved by sodium-dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in 7.5 % or 12.5 % (vol/vol) polyacrylamide gels. .. The gels were transferred to polyvinylidene difluoride (PVDF) membranes, blocked with 5 % skim milk (42590.01, Serva) diluted in PBS-T (1x PBS, 0.1 % Tween 20) and probed overnight at 4 ◦C with sheep anti-cubilin (dil. 1:2000, AF3700, R&D Systems), goat anti-amnionless (dil. 1:2000, AF1860, R&D Systems) or mouse anti-actin (dil. 1:5000.



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    94
    Novus Biologicals control nb820 59231
    Control Nb820 59231, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+nb820+59231/Human+Kidney+Whole+Tissue+Lysate+(Adult+Whole+Normal)/pm41192745-152-12-12
    Average 94 stars, based on 1 article reviews
    control nb820 59231 - by Bioz Stars, 2026-09
    94/100 stars
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